Waters Hplc System Cleaning Procedure

Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.

Waters hplc system cleaning procedure.

Reverse osmosis to remove most contaminants b. Label bottles correctly with bottle content and filling date expiry date. Water flush to clean acid from the system. Maintaining your hplc uhplc uplc systems.

Residues or contaminations may block filters or capillaries. Use only hplc grade solvents and water filtered through 0 2. Water flush to clear customer s mobile phases from the system. 10 nh4oh in water this removes peg and other.

Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307. Controlling contamination in lc ms and hplc ms systems 715001307. Responsibility quality control officer executive. Use solvent inlet filters to protect the system from incoming particles.

3 0 responsibility 3 1 doing. When working with any lc system setting it up and storing it is very important. This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules. The water flushes are only needed to remove the previous solvent from the system.

Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307. To clean the system. No injections are needed with that. Disconnect the column and attach a union and flow directly to waste.

To clean the system. Cleaning decontamination columns general guidelines flow cells general. Ion exchange to remove any remaining ions. Place a b c and d seal wash and needle wash lines into a clean.

When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines. The purification process should include all of the following steps. 2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory. It is necessary to restore the system flow path to a near new level of cleanliness note.

Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa. Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data. Technical assistant 3 2 checking. Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.